Nucleic acid detection and research

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Procedures in Nucleic Acid Research

The rapid development of nucleic acid research has resulted in the emergence of a wide variety of techniques associated with such research. The documentation of even some of these techniques in a well-written form is a formidable task. That the editors of this book have succeeded in producing such a fine first volume is a measure of the care they have taken and the calibre of the specialists co...

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Editorial: Nucleic Acids Research and Nucleic Acid Therapeutics

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Advances in Nucleic Acid Detection and Quantification

The last decade has seen many changes in molecular biology at the bench, as we have moved away from a primary goal of cataloguing genes andmRNA towards techniques that detect and quantify nucleic acid molecules even within single cells. With the invention of the polymerase chain reaction (PCR), a nucleic acid sequence could now be amplified to generate a large number of identical copies, and th...

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Fluorescent hybridization probes for nucleic acid detection.

Due to their high sensitivity and selectivity, minimum interference with living biological systems, and ease of design and synthesis, fluorescent hybridization probes have been widely used to detect nucleic acids both in vivo and in vitro. Molecular beacons (MBs) and binary probes (BPs) are two very important hybridization probes that are designed based on well-established photophysical princip...

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Nucleic acid detection with CRISPR-Cas13a/C2c2.

Rapid, inexpensive, and sensitive nucleic acid detection may aid point-of-care pathogen detection, genotyping, and disease monitoring. The RNA-guided, RNA-targeting clustered regularly interspaced short palindromic repeats (CRISPR) effector Cas13a (previously known as C2c2) exhibits a "collateral effect" of promiscuous ribonuclease activity upon target recognition. We combine the collateral eff...

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ژورنال

عنوان ژورنال: Electroanalysis

سال: 1996

ISSN: 1040-0397,1521-4109

DOI: 10.1002/elan.1140080102